recombinant human rh rankl  (R&D Systems)

Journal: Arthritis Research & Therapy

Article Title: TWEAK and Fn14 expression in the pathogenesis of joint inflammation and bone erosion in rheumatoid arthritis

doi: 10.1186/ar3294

Figure Lengend Snippet: Effect of soluble TWEAK on osteoclast formation and function . Human unfractionated PBMC were cultured for nine days in medium containing rhM-CSF only (25 ng/ml) ( A ), M-CSF and rhTWEAK at 100 ng/ml ( B ), 400 ng/ml ( C ) or 800 ng/ml ( D ), M-CSF and rhRANKL (50 ng/ml) ( E ), or all of M-CSF (25 ng/ml), RANKL (50 ng/ml) and TWEAK (100 ng/ml) ( F ). Cultures were then fixed and stained for TRAP. ( G ) Multinucleated cells (MNC), containing >3 nuclei, positive for TRAP, were counted from quadruplicate wells. Data are expressed as means ± standard deviation. Significant differences were determined by one way analysis of variance (ANOVA) with Tukey post-hoc test: a indicates difference to M-CSF only control ( P < 0.001) and b denotes difference to M-CSF+RANKL treatment ( P < 0.05). (H) PBMC were seeded onto dentine slices in the presence of rhM-CSF (25 ng/ml) with the addition of rhRANKL (50 ng/ml) and/or rhTWEAK as indicated. Resorption was assessed after 14 days of culture by SEM and is expressed as the mean ± SD resorption expressed as a percentage of that measured for the RANKL/M-CSF control. Data shown are pooled from two independent experiments with resorption assessed for four dentine slices/treatment/donor. No significant differences were observed between RANKL treatments.

Article Snippet: Murine RAW 264.7 cells were plated at 1 × 10 5 cells/well into 96-well plates, in the presence or absence of recombinant human (rh) Receptor Activator of Nuclear factor Kappa-B Ligand (RANKL) (100 ng/ml) (Millipore) and/or rhTWEAK (10 to 800 ng/ml).

Techniques: Cell Culture, Staining, Standard Deviation, Control

Journal: Arthritis Research & Therapy

Article Title: TWEAK and Fn14 expression in the pathogenesis of joint inflammation and bone erosion in rheumatoid arthritis

doi: 10.1186/ar3294

Figure Lengend Snippet: Effect of soluble TWEAK on cell surface RANKL expression by STRO-1 osteoblast subpopulation . Human primary osteoblasts were cultured untreated or treated with rhTWEAK (50 ng/ml) for three days, harvested by collagenase/dispase digestion and stained for STRO-1 and RANKL expression. Stained cells were analysed by FACS. The percentage of cells present in each of the four sub-populations is indicated. Data are presented for two independent donors' cells.

Article Snippet: Murine RAW 264.7 cells were plated at 1 × 10 5 cells/well into 96-well plates, in the presence or absence of recombinant human (rh) Receptor Activator of Nuclear factor Kappa-B Ligand (RANKL) (100 ng/ml) (Millipore) and/or rhTWEAK (10 to 800 ng/ml).

Techniques: Expressing, Cell Culture, Staining